AADH Induces Substrate Conformation Crucial For Promoting Vibration That Reduces The Effective Potential Energy Barrier To Proton Transfer

Main Category: Biology / Biochemistry
Article Date: 15 May 2008 - 1:00 PDT

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The role of promoting vibrations in enzymic reactions involving hydrogen tunnelling is contentious. Whilemodels incorporating such promoting vibrations have successfully reproduced and explained experimental observations, it has also been argued that such vibrations are not part of the catalytic effect.

In this study,we have employed combined quantum mechanical/molecular mechanical methods with molecular dynamics and potential energy surface calculations to investigate how enzyme and substratemotion affects the energy barrier to proton transfer for the rate-limiting H-transfer step in aromatic amine dehydrogenase (AADH) with tryptamine as substrate.

In particular, the conformation of the iminoquinone adduct induced by AADH was found to be essential for a promoting vibration identified previously-this lowers significantly the 'effective' potential energy barrier, that is the barrier which remains to be surmounted following collective, thermally equilibrated motion attaining a quantum degenerate state of reactants and products.

When the substrate adopts a conformation similar to that in the free iminoquinone, this barrier was found to increase markedly. This is consistent with AADH facilitating the H-transfer event by holding the substrate in a conformation that induces a promoting vibration.

Journal of the Royal Society Interface

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Journal of the Royal Society Interface