Thermo Fisher Scientific Improves Workflow In HLA Laboratories With 10-Second Analysis Of DNA Microsamples Using Nanodrop 1000

Main Category: Medical Devices / Diagnostics
Also Included In: Genetics;  Biology / Biochemistry
Article Date: 31 Jul 2008 - 15:00 PDT

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Thermo Fisher Scientific Inc., the world leader in serving science, will exhibit a new spectrophotometer that offers unique benefits for human leukocyte antigen (HLA) typing laboratories dealing with donor tissue matching. The Thermo Scientific NanoDrop™ 1000 represents the growing trend of successful genome research tools finding their way to the clinical research setting. Specifically, the NanoDrop 1000 is utilized in the workflow as a replacement to time-consuming traditional spectrophotometers where efficiency and time-to-result are critical.

The NanoDrop 1000 is capable of carrying out full UV-Vis absorbance measurements with only 1 ul of undiluted sample, enabling HLA laboratories to significantly speed up analysis time. The NanoDrop 1000 has been integrated into tissue typing workflows through a validation process as set by the HLA typing community. The NanoDrop 1000 spectrophotometer will be showcased for the first time at the Thermo Scientific booth 2739 at AACC 2008, being held in Washington D.C., from July 29 - 31.

This innovative cuvetteless method enables HLA laboratories to quickly and effectively process DNA samples without compromising yield. HLA is the major histocompatibility complex in humans, and laboratories performing HLA typing have distinct requirements for instrumentation, including preserving sample yield and optimizing analysis time. The NanoDrop 1000 spectrophotometer has been successfully integrated into the HLA typing workflow in a number of leading laboratories. DNA is extracted and purified from donor tissue and quantified on the NanoDrop 1000 using a patented sample retention system requiring only 1 ul of sample. This quantitation method enables HLA laboratories to quickly and effectively process DNA samples without compromising yield.

In many HLA cases, donor workup results are required in less than four hours as the speed and accuracy of tissue analysis can affect the long-term graft survival of the tissue. The NanoDrop 1000 spectrophotometer allows laboratories to analyze microsamples of DNA in only 10 seconds, reducing labor time and increasing laboratory productivity. Additionally, the novel microsample quantitation technology of the NanoDrop 1000 is critical for typing samples of limited cell mass, such as mononucleated bone marrow specimens.

In addition to reducing spectrophotometric quantitation prior to DNA amplification, the NanoDrop 1000 enables HLA typing laboratories to comply with the strict standards set by the ASHI. The NanoDrop 1000 has passed the rigorous validation process for HLA laboratories across the United States, and is now recommended by many certified laboratory inspectors.

Notes

For more information about the Thermo Scientific NanoDrop 1000 spectrophotometer, please visit the Thermo Scientific booth 2739 at AACC 2008. Alternatively please email nanodrop@thermofisher.com, call +1 302-479-7707 or visit http://www.nanodrop.com.

Spectrophotometers are general purpose laboratory instruments. They have not been cleared or approved by the United States Food and Drug Administration, the European IVD Directive or any other agency for diagnostic, clinical or other medical use.

Thermo Scientific is part of Thermo Fisher Scientific Inc. the world leader in serving science.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, enabling our customers to make the world healthier, cleaner and safer. With annual revenues of $10 billion, we have more than 30,000 employees and serve over 350,000 customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as environmental and industrial process control settings. Serving customers through two premier brands, Thermo Scientific and Fisher Scientific, we help solve analytical challenges from routine testing to complex research and discovery. Thermo Scientific offers customers a complete range of high-end analytical instruments as well as laboratory equipment, software, services, consumables and reagents to enable integrated laboratory workflow solutions. Fisher Scientific provides a complete portfolio of laboratory equipment, chemicals, supplies and services used in healthcare, scientific research, safety and education. Together, we offer the most convenient purchasing options to customers and continuously advance our technologies to accelerate the pace of scientific discovery, enhance value for customers and fuel growth for shareholders and employees alike.

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