Immune Responses In Disease Monitored

Main Category: Immune System / Vaccines
Also Included In: Diabetes;  Blood / Hematology;  Biology / Biochemistry
Article Date: 05 Sep 2008 - 0:00 PDT

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A recent study (doi:10.1016/j.clim.2008.06.009) published in Clinical Immunology, the official journal of the Clinical Immunology Society (CIS), describes a new method enabling the detection of multiple parameters of single human cells. The report demonstrates the characterization of specific blood cells from an individual with type 1 diabetes, providing information about the role these cells might play in the development of the disease and during therapy.

Classification of blood cells, including B and T cells, is important for distinguishing immune responses to pathogens, allergens, or self-antigens in autoimmune diseases. Although various techniques are available to identify cell surface determinants, cytokines and antibodies secreted by blood cells, so far it has not been possible to study multiple secreted proteins while also assigning surface displayed markers to individual living cells.

A collaborative group of investigators from Harvard Medical School and Massachusetts Institute of Technology in the USA, describe how a combination of existing and enhanced immunological methods can identify and characterize rare B cells from blood of a recent onset type 1 diabetic subject.

"Although this is a small pilot study, it is a useful proof of principle for single cell interrogation methodology, which is potentially of general utility", according to immunologist Gerald Nepom from the University of Washington, School of Medicine in Seattle, USA in his commentary published in the same issue of Clinical Immunology (http://www.elsevier.com/locate/yclim).

"This article describes a very exciting new immunodiagnostic tool, potentially enabling the discovery of novel biomarkers for the pathogenesis of immunologic disorders and in monitoring therapy", said Andy Saxon", the Editor-in-Chief of the CIS journal.

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Article adapted by Medical News Today from original press release.
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E.M. Bradshaw, S.C. Kent, V. Tripuraneni, T. Orban, H.L. Ploegh, D.A. Hafler, J.C. Love, Concurrent detection of secreted products from human lymphocytes by microengraving: Cytokines and antigen-reactive antibodies, 2008 Clinical Immunology, published by Elsevier, In press (available on-line).

About CIS

The Clinical Immunology Society (CIS) is an international professional organization which includes more than 750 clinicians, investigators and trainees. The mission of the CIS is to facilitate education, translational research and novel approaches to therapy in clinical immunology to promote excellence in the care of patients with immunologic / inflammatory disorders (http://www.clinimmsoc.org/).

About Elsevier

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Elsevier (http://www.elsevier.com/) is a global business headquartered in Amsterdam, The Netherlands and has offices worldwide. Elsevier is part of Reed Elsevier Group plc (http://www.reedelsevier.com/), a world-leading publisher and information provider. Operating in the science and medical, legal, education and business-to-business sectors, Reed Elsevier provides high-quality and flexible information solutions to users, with increasing emphasis on the Internet as a means of delivery. Reed Elsevier's ticker symbols are REN (Euronext Amsterdam), REL (London Stock Exchange), RUK and ENL (New York Stock Exchange).

Source: Floris de Hon
Elsevier