News From The Journal Of Neuroscience

Main Category: Neurology / Neuroscience
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Article Date: 24 Sep 2008 - 6:00 PDT

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1. Synaptic Properties of Corticothalamic Synapses

Alexander Groh, Christiaan P. J. de Kock, Verena C. Wimmer, Bert Sakmann, and Thomas Kuner

In rodents, neurons in layer 5 of somatosensory (barrel) cortex project to, and form giant terminals on, neurons in the posterior medial thalamus. With such long-range connections, identifying synaptically coupled neurons in which to study synaptic properties is difficult. Groh et al. cleverly overcame this problem by virally expressing a fluorescent protein in rat cortical neurons, which enabled visualization of giant terminals in corticothalamic slice cultures. Whole-cell patches were used both to fill postsynaptic neurons with dye and to record responses to extracellular stimulation of presynaptic terminals. Single presynaptic spikes produced large EPSCs and triggered multiple action potentials in postsynaptic cells. But the spikes also produced rapid short-term depression, so subsequent stimuli did not generate postsynaptic action potentials unless the stimulus frequency was <2 Hz. Therefore, stimuli that mimicked spontaneous activity recorded in vivo (typically 4-6 Hz) elicited postsynaptic spikes only after occasional periods of silence or when two presynaptic terminals were stimulated simultaneously.

2. TGF-β1, a Schwann-Cell-Derived Synaptogenic Molecule

Zhihua Feng and Chien-Ping Ko

Extensive research on the neuromuscular junction has produced a detailed understanding of the interactions that occur between neurons and muscles during synaptic development. One essential step in synaptogenesis is secretion of agrin by nerve terminals, which induces clustering of acetylcholine receptors (AChRs) in the muscle. Nonmyelinating, perisynaptic Schwann cells also contribute to formation of the neuromuscular junction, by guiding axonal growth and by secreting another factor that promotes AChR clustering. This week, Feng and Ko identify the Schwann-cell-derived synaptogenic factor as transforming growth factor β1 (TGF-β1). In frog nerve-muscle cocultures, TGF-β1 replicated the ability of Schwann-cell-conditioned media to increase AChR clustering in muscles. Furthermore, depletion of TGF-β1 abolished the synaptogenic effect of the conditioned media. TGF-β1 did not induce AChR clustering in pure muscle cultures - which is unsurprising, because muscle lacks TGF-β1 receptors. Instead, TGF-β1 appears to exert its synaptogenic effect by inducing agrin secretion by the growing nerve.

3. Precise and Imprecise Olfactory Coding

Derek J. Hoare, Catherine R. McCrohan, and Matthew Cobb

How olfactory information is processed by the nervous system remains a mystery. Drosophila larvae provide a good model system for studying olfactory processing, because they have only 25 odor receptor (OR) genes and 21 pairs of olfactory sensory neurons (OSNs), and Drosophila OSNs, like those in other organisms, express a single OR gene. It has long been assumed that OSNs respond in a characteristic way to particular odors. To test this hypothesis, Hoare et al. generated several strains of Drosophila larvae that each expressed a single functional OSN. As expected, most OSNs responded in a qualitatively consistent, characteristic manner (excitation, inhibition, or no response) to different odors. Surprisingly, however, ~20% of OSNs had variable responses to particular odors, responding only sometimes, independent of concentration or duration of the stimulus. Such stochastic responses were also detected in wild-type larvae. How such stochastic coding might aid olfactory discrimination is a new puzzle to be solved.

4. Damage Response in the Retinal Pigment Epithelium

Amir Rattner, Leila Toulabi, John Williams, Huimin Yu, and Jeremy Nathans

The retinal pigment epithelium (RPE), which lies adjacent to the outer segments of photoreceptors, is essential for retinal health and function, providing nutrients, maintaining ionic balance, and converting all-trans retinol back to 11-cis retinal during the visual cycle. Local degradation of the RPE is a common cause of vision loss in age-related macular degeneration, and mutations in RPE proteins can lead to retinal degeneration and congenital blindness. To understand the changes that occur in the RPE in response to damage, Rattner et al. examined gene expression changes that occur following bright light exposure and retinal detachment. These two types of damage produced largely overlapping patterns of gene expression changes. Notably, genomic changes in the RPE appeared to be triggered by a signal from the retina, because the changes did not occur in an ex vivo preparation after the retina had been removed.

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Article adapted by Medical News Today from original press release.
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Source: Sara Harris
Society for Neuroscience