Is Acute Cellular DNA Damage Induced By HBV Infection?

Main Category: Liver Disease / Hepatitis
Also Included In: Genetics
Article Date: 13 Nov 2008 - 1:00 PST

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Eukaryotic cells employ multiple strategies of checkpoint signaling and DNA repair mechanisms to monitor and repair damaged DNA. There are two branches of the checkpoint response pathway, ataxia telangiectasia-mutated (ATM) pathway and ATM-Rad3-related (ATR) pathway. Virus replication presents the host cells with large amounts of exogenous genetic material, including DNA ends and unusual structures. Therefore, infected cells recognize viral replication as a DNA damage stress and elicit DNA damage signal transduction, which ultimately induces apoptosis as part of host immune surveillance. There was no evidence so far that the ATM/ATR kinases or their downstream pathways are triggered by HBV infection.

A research article published in the World Journal of Gastroenterology addresses this question. This research group was leaded by Dr Hui Zhong from Beijing Institute of Biotechnology, China.

Using HL7702 hepatocytes with HBV-positive serum as material, they evaluate protein expression levels in HBV infected cells or in non-infected cells; immunofluorescence to show ATR foci ands Chk1 phosphorylation foci formation; flow cytometry to analyze the cell cycle and apoptosis; ultraviolet radiation (UV) and ionizing radiation (IR) treated cells to mimic DNA damage; Trypan blue staining to count the viable cells.

They found that HBV infection induced increased steady state of ATR protein and increased phosphorylation of multiple downstream targets including Chk1, p53 and H2AX. In contrast to ATR and its target, the phosphorylated form of ATM at Ser-1981 and its downstream substrate Chk2 phosphorylation at Thr-68 did not visibly increase upon infection. However, the level of Mre11 and p21 were reduced beginning at 0.5 h after HBV-positive serum addition. Also, HBV infection led to transient cell cycle arrest in the S and the G2 phases without accompanying increased apoptosis. Research on analyzing cell survival change upon radiation followed HBV infection showed that survival of UV treated host cells was greatly increased by HBV infection, owing to the reduced apoptosis. Meanwhile, survival of IR treated host cells was reduced by HBV infection.

Their result indicated that HBV induces cellular DNA damage response dependent on ATR but escapes the consequences of activation of the DNA damage checkpoint by degradation of checkpoint proteins on different levels. The implication of this is that with time, persistent HBV infection may lead to the accumulation of a variety of mutations which would ultimately give rise to HCC.

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Article adapted by Medical News Today from original press release.
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Reference: Zhao F, Hou NB, Yang XL, He X, Liu Y, Zhang YH, Wei CW, Song T, Li L, Ma QJ, Zhong H. Ataxia telangiectasia-mutated-Rad3-related DNA damage checkpoint signaling pathway triggered by hepatitis B virus infection. World J Gastroenterol 2008; 14(40): 6163-6170 http://www.wjgnet.com/1007-9327/14/6163.asp

Correspondence to: Hui Zhong, PhD, Molecular genetics department Beijing Institute of Biotechnology, Beijing 100850, China.

About World Journal of Gastroenterology

World Journal of Gastroenterology (WJG), a leading international journal in gastroenterology and hepatology, has established a reputation for publishing first class research on esophageal cancer, gastric cancer, liver cancer, viral hepatitis, colorectal cancer, and H pylori infection and provides a forum for both clinicians and scientists. WJG has been indexed and abstracted in Current Contents/Clinical Medicine, Science Citation Index Expanded (also known as SciSearch) and Journal Citation Reports/Science Edition, Index Medicus, MEDLINE and PubMed, Chemical Abstracts, EMBASE/Excerpta Medica, Abstracts Journals, Nature Clinical Practice Gastroenterology and Hepatology, CAB Abstracts and Global Health. ISI JCR 2003-2000 IF: 3.318, 2.532, 1.445 and 0.993. WJG is a weekly journal published by WJG Press. The publication dates are the 7th, 14th, 21st, and 28th day of every month. WJG is supported by The National Natural Science Foundation of China, No. 30224801 and No. 30424812, and was founded with the name of China National Journal of New Gastroenterology on October 1, 1995, and renamed WJG on January 25, 1998.

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The WJG Press mainly publishes World Journal of Gastroenterology.

Source: Lai-Fu Li
World Journal of Gastroenterology