Thermo Scientific DNA Polymerases And Master Mixes Improve Yield And Specificity In PCR Amplifications

Main Category: Pharma Industry / Biotech Industry
Article Date: 15 Jul 2009 - 1:00 PDT

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Thermo Fisher Scientific Inc., the world leader in serving science, announced it offers a premium collection of Thermo Scientific Taq DNA polymerases that are licensed and optimised for use in PCR processes. ThermoPrime Taq DNA Polymerase and Thermo-Start® Taq DNA Polymerase are designed to provide excellent amplification specificity and improved product yield. In addition, the high purity of these enzymes ensures their consistent and reliable performance.

ThermoPrime Taq DNA Polymerase exhibits enhanced thermal stability at DNA denaturation temperatures, ensuring optimal enzyme activity throughout the thermal cycling process and maximum product yield. Suitable for the amplification of templates up to 4kb in length, this high performance enzyme is ideal for both standard and long PCR applications.

Thermo-Start DNA Polymerase is a chemically modified version of ThermoPrime Taq DNA Polymerase, developed to ensure high specificity in hot-start PCR applications. Unlike other hot-start DNA polymerases, the modification in Thermo-Start DNA Polymerase is extremely stable (requiring heating at 95ºC for 15 minutes for activation) and results in sustained activity of the enzyme over an extended number of cycles with high denaturation temperatures. This eliminates non-specific amplification and increases product yield.

These ultra pure enzymes are also available in convenient master mix formats for quick, easy and reproducible setup of reagents - ideal for high throughput applications.

Dr. Justin Pachebat of the Genome Mapping and Sequencing facility at the University of Cambridge, UK, said, "We perform high throughput PCR typing for physical genome mapping, setting up 5,000 reactions per run in 384-well plates. In a direct comparison with our previous PCR mix, using the same reaction conditions, we found the Thermo-Start PCR Master Mix to be highly suitable for high-throughput/robotic PCR applications, giving us remarkably better results."

The ThermoPrime and Thermo-Start PCR Master Mixes are also available with ReddyMix™, a unique PCR Buffer that is specially formulated with a red tracker dye to allow direct loading of the PCR product onto gel. For a full product listing and further information visit http://www.thermo.com/gen.

Source
Thermo Fisher Scientific