Synthesis-Dependent Strand Annealing In Meiosis

Main Category: Biology / Biochemistry
Also Included In: Genetics
Article Date: 05 Nov 2007 - 17:00 PDT

email to a friend   printer friendly   view / write opinions   rate article

In organisms that reproduce sexually, sex cells (gametes) are produced by the specialized cell division called meiosis, which halves the number of chromosomes from two sets (diploid) to one (haploid). During meiosis, homologous DNA molecules exchange genetic material (in a process called homologous recombination), thereby contributing to genetic diversity. In addition, a subset of recombinants, called crossovers, creates connections between chromosomes that are required for those chromosomes to be accurately segregated.

Accurate segregation ensures that gametes contain one and only one copy of each chromosome. Recombination is initiated by chromosome breakage. A regulatory process then selects a subset of breaks to be healed by a mechanism that forms crossover recombinants. Many of the remaining breaks are healed to form so-called "noncrossover" recombinants (also referred to as "gene conversions"). Until recently it was thought that both crossovers and noncrossovers were formed by nearly identical pathways; which form arose was thought to depend on how the last enzyme in the pathway attacked the last DNA intermediate.

However, more recent observations suggested noncrossover recombinants might arise by a mechanism involving less stable intermediates than those required to make crossovers. In this week's issue of PLoS Biology Dr. Douglas Bishop, Dr. Melissa McMahill, and Dr. Caroline Sham show how a yeast strain was constructed that allowed detection of a genetic signature of such unstable recombination intermediates. This strain provided evidence that meiotic crossovers and non-crossovers do indeed form by quite different mechanisms.

Citation: McMahill MS, Sham CW, Bishop DK (2007) Synthesis-dependent strand annealing in meiosis. PLoS Biol 5(11): e299. doi:10.1371/journal.pbio.0050299
Please click here

http://www.plosbiology.org

Public Library of Science
185 Berry Street, Suite 3100
San Francisco, CA 94107
USA