Is OB Glue Paste Technique Ideal To Establish Nude Mouse Transplantation Model?

Main Category: GastroIntestinal / Gastroenterology
Also Included In: Cancer / Oncology;  Biology / Biochemistry;  Transplants / Organ Donations
Article Date: 22 Sep 2008 - 1:00 PDT

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Establishment of human gastric cancer nude mouse transplantation models has undergone three representative stages: subcutaneous transplantation of cell suspension, gastric wall seeding of cell suspension, and intra-gastric wall transplantation of histologically intact tissue, of which the fresh tissue orthotopic transplantation model has two types: the "suture method"and the "gastric bursa method".But the "suture method"or the "gastric bursa method" transplantation has many practical difficulties and disadvantages in establishing the model; for example, the tumor tissue is easy to fall off postoperatively; manipulation is complex and needs relatively high skills; there may be great blood loss during suturing, and mortality is relatively high.

A research article published on August 14, 2008 in the World Journal of Gastroenterology addresses this question. The research team led by Prof. Xu from Shanghai Changzheng Hospital. The present study used OB glue paste technique to establish orthtopic transplantation models by implanting SGC-7901 and MKN-45 human gastric cancer cell strains into the gastric wall of nude mice. Biological features, growth of the implanted tumors, the success rate of transplantation and the rate of auto metastasis of the two models were observed.

Observations showed that the success rate of orthotopic transplantation, rate of hepatic metastasis, rate of pulmonary metastasis, rate of peritoneal metastasis, rate of lymphocytic metastasis, rate of splenic metastasis, and the occurrence of ascites of SGC-7901 and MKN-45 models were 97% and 96%; 42.13% and 94.20%; 48.43% and 57.97%; 30.83% and 36.96%; 67.30% and 84.06%, 59.75% and 10.53%, and 47.80% and 36.96%, respectively.Although different tumor strains grew at different rates, infiltrative growth and multi-organ metastases are common features of the two models, and these features are similar to the clinical presentation of invasive metastasis. Chromosomal identification also demonstrated that both orthotopic and metastatic tumors came from the human gastric cancer implanted. This technique is an ideal means of model establishment owing to its easier manipulation, shorter operating time, less blood loss, quicker postoperative recovery, higher survival of experimental animals and avoidance of tumor falling off.

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Reference: Shi J, Wei PK, Zhang S, Qin ZF, Li J, Sun DZ, Xiao Y, Yu ZH, Lin HM, Zheng GJ, Su XM, Chen YL, Liu YF, Xu L. OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models. World J Gastroenterol 2008; 14(30): 4800-4804 http://www.wjgnet.com/1007-9327/14/4800.asp

Correspondence to: Associate Professor Ling Xu, Department of Traditional Chinese Medicine, Shanghai Changzheng Hospital, Shanghai 200003, China.

About World Journal of Gastroenterology

World Journal of Gastroenterology (WJG), a leading international journal in gastroenterology and hepatology, has established a reputation for publishing first class research on esophageal cancer, gastric cancer, liver cancer, viral hepatitis, colorectal cancer, and H pylori infection. It provides a forum for both clinicians and scientists. WJG has been indexed and abstracted in Current Contents/Clinical Medicine, Science Citation Index Expanded (also known as SciSearch) and Journal Citation Reports/Science Edition, Index Medicus, MEDLINE and PubMed, Chemical Abstracts, EMBASE/Excerpta Medica, Abstracts Journals, Nature Clinical Practice Gastroenterology and Hepatology, CAB Abstracts and Global Health. ISI JCR 2003-2000 IF: 3.318, 2.532, 1.445 and 0.993. WJG is a weekly journal published by WJG Press. The publication dates are the 7th, 14th, 21st, and 28th of every month. The WJG is supported by The National Natural Science Foundation of China, No. 30224801 and No. 30424812, and was founded with the title China National Journal of New Gastroenterology on October 1, 1995, and renamed WJG on January 25, 1998.

Source:Lin-Lin Xiao
World Journal of Gastroenterology

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