Skin Cells Reprogrammed Into Stem Cells In Mouse And Monkey Models

Main Category: Stem Cell Research
Also Included In: Biology / Biochemistry
Article Date: 04 Dec 2008 - 5:00 PDT

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Scientists have successfully created the first induced pluripotent stem (iPS) cell lines from adult monkey skin cells. The research, published by Cell Press in the December issue of the journal Cell Stem Cell, demonstrates that the method of direct reprogramming is conserved among species and may be useful for creation of clinically valuable primate models for human diseases.

Although previous work has shown that induction of four key transcription factors can reprogram adult mouse and human skin cells into iPS cells, creation of iPS cells in other species has not been demonstrated. "We sought to generate monkey iPS cells from skin cells isolated an adult male rhesus macaque using the predicted monkey transcription factors OCT4, SOX2, KLF4 and c-MYC," explains Dr. Hongkui Deng from the Key Laboratory of Cell Proliferation and Differentiation at Peking University in Beijing, China.

Dr. Deng and colleagues used retroviruses expressing these four factors to infect adult monkey skin cells. This technique led to creation of cells which displayed multiple hallmarks of embryonic stem (ES) cells. Specifically, the cells exhibited physical characteristics associated with ES cells, expressed genes appropriate for ES cells and possessed the ability to develop into multiple types of differentiated cells. These results reveal that monkey iPS cells can be generated using the same four transcription factors that have been used to successfully create mouse and human iPS cells.

The work has multiple exciting applications. "As the rhesus macaque is the most relevant primate model for most human diseases, highly efficient generation of monkey iPS cells would allow investigation of the treatment of various diseases in this model," offers Dr. Deng. "In addition, direct reprogramming with the four transcription factors could be a universal strategy for generating iPS cells in other species."

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The researchers include Haisong Liu, Peking University, Beijing, China, Shenzhen Graduate School of Peking University, Shenzhen, China; Fangfang Zhu, Peking University, Beijing, China, Shenzhen Graduate School of Peking University, Shenzhen, China; Jun Yong, Peking University, Beijing, China, Shenzhen Graduate School of Peking University, Shenzhen, China; Pengbo Zhang, Peking University, Beijing, China; Pingping Hou, Peking University, Beijing, China;Honggang Li, Peking University, Beijing, China; Wei Jiang, Peking University, Beijing, China; Jun Cai, Peking University, Beijing, China; Meng Liu, Peking University, Beijing, China, Shenzhen Graduate School of Peking University, Shenzhen, China; Kai Cui, Peking University, Beijing, China; Xiuxia Qu, Peking University, Beijing, China;Tingting Xiang, Peking University, Beijing, China; Danyu Lu, Peking University, Beijing, China; Xiaochun Chi, Peking University, Beijing, China; Ge Gao, Peking University, Beijing, China; Weizhi Ji, Chinese Academy of Sciences, Kunming, China; Mingxiao Ding, Peking University, Beijing, China; and Hongkui Deng, Peking University, Beijing, China, Shenzhen Graduate School of Peking University, Shenzhen, China.

Source: Cathleen Genova
Cell Press

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