Metagene 'Portraits' Used By UC San Diego Researchers To Reveal Distinct Stages Of Kidney Formation
Now, a multi-disciplinary team at UC San Diego has used novel computational methods to gain insight into kidney formation. By performing time-series analyses of global gene expression, and clustering these genes into larger groups known as metagene portraits and also calculating entropy values, the researchers have identified distinct stages of organ formation.
The research, published in the December 9 edition of Science Signaling, is also significant in that it suggests additional genes that had not been previously implicated in orchestration of kidney development.
"The approach may also be helpful for understanding tissue regeneration and pathological states," said Sanjay K. Nigam, Professor of UC San Diego's Department of Pediatrics, Department of Medicine (Nephrology-Hypertension) and the Department of Cellular and Molecular Medicine. "Analysis of the metagene portraits suggested points of stability and transition which are not as evident using conventional methods."
Additional research members in the study include Igor F. Tsigelny, a project scientist with UC San Diego's Department of Chemistry and Biochemistry and the San Diego Supercomputer Center at UC San Diego; and Valentina L. Kouznetsova; Derina E. Sweeney; Wei Wu; and Kevin T. Bush; all with UC San Diego's School of Medicine.
In efforts to provide a more comprehensive view of organogenesis, the researchers compared and analyzed visual representations of gene expression patterns during kidney development. Approximately 30,000 genes were organized into 650 groups called metagenes, or gene clusters known as self-organizing maps (SOMs).
Essentially acting as unsupervised learning neural networks, SOMs cluster genes with similar temporal expression profiles into metagene portraits. In this case, the SOMs were collected using microarray gene expression data and a high-dimensional data analysis program called Gene Expression Dynamics Inspector, or GEDI. UC San Diego scientists then performed a detailed time-series analysis of the kidney SOMs reflecting various stages of organogenesis. That was followed by entropy calculations for each SOM to measure the differences in states during the various stages, before correlating these results with morphometric parameters and specific gene networks.
Taken together, analysis of the metagene portraits suggested that kidney formation could be divided into as many as eight distinct stages.
"Although there is morphological support for the notion of stages, the beginning and end points of those stages can be difficult to define because many basic morphogenetic processes occur simultaneously," said Tsigelny. "Our research suggests that with the metagene analysis and entropy calculations, global gene expression can be used to more clearly define these stages and allow us to have a fuller understanding about how organs form."
Research for this UC San Diego study was supported by grants from the National Institutes of Health. For the full research article in Science Signaling, please see: http://stke.sciencemag.org/cgi/content/full/1/49/ra16
Source: Jan Zverina
University of California - San Diego
There are no references listed for this article.
Please use one of the following formats to cite this article in your essay, paper or report:
Zverina, Jan. "Metagene 'Portraits' Used By UC San Diego Researchers To Reveal Distinct Stages Of Kidney Formation." Medical News Today. MediLexicon, Intl., 12 Dec. 2008. Web.
29 Mar. 2017. <http://www.medicalnewstoday.com/releases/132872.php>
Zverina, J. (2008, December 12). "Metagene 'Portraits' Used By UC San Diego Researchers To Reveal Distinct Stages Of Kidney Formation." Medical News Today. Retrieved from
Please note: If no author information is provided, the source is cited instead.
Contact our news editors
For any corrections of factual information, or to contact our editorial team, please see our contact page.
Copyright Medical News Today: Excluding email/sharing services explicitly offered on this website, material published on Medical News Today may not be reproduced, or distributed without the prior written permission of Medilexicon International Ltd. Please contact us for further details.