The video explains that the calorie content of food was determined in the late 1800s by chemist Wilbur O. Atwater. Atwater built a four-by-eight-foot device called a respiration calorimeter, which was big enough to allow a person to step into it. It measured the amount of heat they released, the amount of oxygen they consumed and the carbon dioxide they gave off after eating a variety of foods.
Using this device, Atwater was able to measure the precise amount of energy contained in thousands of food items. He found that carbohydrates and proteins were worth 4 calories per gram and fats about 9 calories per gram, which is about 1/28th of an ounce. This 4-9-4 rule remains embodied in today's Nutrition Facts Labels.
The video, from the award-winning Digital Services Unit in the ACS Office of Public Affairs, is based on an article in the latest issue of ChemMatters, ACS' quarterly magazine for high school students.