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The water-soluble metabolite profile of human mesenchymal stem cells is known, but the lipid profile remains unclear.
Haiyang Dai and colleagues from Shantou University Medical College used methanol-chloroform and perchloric acid to extract lipid-soluble metabolites and water-soluble metabolites, respectively. Furthermore, a dual phase extraction method using methanol-chloroform and water was used to obtain both water and lipid fractions simultaneously.
Among the different extraction procedures, perchloric acid was more efficient in extracting water-soluble metabolites and methanol-chloroform was efficient in extracting organic components compared with the dual phase extraction method. Nuclear magnetic resonance spectroscopy was found to be highly sensitive for analyzing lipid-soluble extracts.
The researchers confirm that soluble metabolite spectrum has an important role to reflect lipid metabolic changes in the cytoplasm and cell membrane, a comprehensive analysis of spectral characteristics of water- and lipid-soluble metabolite extracts from mesenchymal stem cells provides a more comprehensive understanding of their metabolic characteristics, in order to achieve the best detection efficiency under the condition of reducing cell usage.
These findings were published in the Neural Regeneration Research (Vol. 8, No. 22, 2013).
The following image shows a typical 1H-nuclear magnetic resonance spectrum of the lipid-soluble metabolites of human umbilical cord mesenchymal stem cells. The concentration of glutamate extracted with the dual phase extraction method (lower) was higher than that obtained with the methanol-chloroform method (upper):
Dai HY, Hong BK, Xu ZF, Ma L, Chen YW, Xiao YY, Wu RH. Nuclear magnetic resonance spectroscopy is highly sensitive for lipid-soluble metabolites. Neural Regen Res. 2013;8(22):2103-2110.
Article adapted by Medical News Today from original press release. Click 'references' tab above for source.
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