Molecular markers used for assessment of early sciatic nerve injury
Substance P and calcitonin gene-related peptide are the main neuropeptides in peripheral nerve ganglia, which can anterogradely transmit nociceptive information to the central nervous system.
In a study by Dr. Changma Fu and co-workers from First Hospital of Anhui Medical University in China, a model of sciatic nerve defect was prepared by dissecting the sciatic nerve at the middle, left femur in female Sprague Dawley rats. The two ends of the nerve were encased in a silica gel tube. L5 dorsal root ganglia were harvested 7, 14 and 28 days post sciatic nerve injury for immunohistochemical staining.
Results showed that substance P and calcitonin gene-related peptide expression increased significantly in dorsal root ganglion of rats with sciatic nerve injury. This increase peaked at 7 days, declined at 14 days, and reduced to normal levels by 28 days post injury.
These findings published in the Neural Regeneration Research (Vol. 8, No. 33, 2013) suggest that these neuropeptides may possibly serve as an index for evaluating early peripheral nerve injury.
Substance P and calcitonin gene-related peptide expression in dorsal root ganglia in sciatic nerve injury rats, Fu CM, Yin ZS, Yu DF, Yang ZH., Neural Regen Res. 2013;8(33):3124-3130
Image credit: Neural Regeneration Research