After central nervous system injury, the increase in Nogo protein is mediated by Nogo-66 receptor gene, leading to the collapse of growth cones and inhibiting the extension of neuronal neurites.
Hematoxylin-eosin staining revealed that after transplantation of Nogo-66 receptor gene-silenced bone marrow mesenchymal stem cells in rats with spinal cord injury, astrocytes exhibited reactive hypertrophy, aggregated and formed scars at the edge of the affected site, some cells were spindle-shaped, forming a dense network with their processes, but cavities disappeared.
Credit: Neural Regeneration Research
Zhiyuan Li and his team, Heibei Xingtai People's Hospital, China used RNA interference to silence Nogo-66 receptor gene expression in bone marrow mesenchymal stem cells (BMSCs), blocked the inhibitory effect of Nogo protein, promoted the process growth of neurons differentiated by transplanted cells, improved the therapeutic effects and increased the success rate of BMSCs transplantation for repair of central nerve system injuries.
These results indicate that Nogo-66 receptor gene plays an important role in secondary nerve injury.
These results were published in Neural Regeneration Research (Vol. 9, No. 8, 2014).
Article: " Bone marrow mesenchymal stem cells with Nogo-66 receptor gene silencing for repair of spinal cord injury," Zhiyuan Li1, Zhanxiu Zhang1, Lili Zhao1, Hui Li1, Suxia Wang1, Yong Shen2 (1 Department of Joint Orthopedics, Hebei Provincial Xingtai People's Hospital, Xingtai, Hebei Province, China; 2 Department of Spinal Orthopedics, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China)
Li ZY, Zhang ZX, Zhao LL, Li H, Wang SX, Shen Y. Bone marrow mesenchymal stem cells with Nogo-66 receptor gene silencing for repair of spinal cord injury. Neural Regen Res. 2014;9(8):806-814.