The over-expression of α-synuclein is a major factor in the death of dopaminergic neurons in a methamphetamine-induced model of Parkinson's disease (PD).
After α-synuclein knockdown by stereotaxic injection of α-synuclein into the right striatum, the number of apoptotic cells in the striatum of rat models of mtamfetamine-induced Alzheimer's disease was significantly reduced, as shown by TUNEL staining.
Credit: Neural Regeneration Research
Dr. Huijun Wang, School of Basic Medical Sciences, Southern Medical University, China and his team injected α-synuclein-shRNA lentivirus stereotaxically into the right striatum of experimental rats to inhibit α-synuclein mRNA and protein expression.
Results showed that after α-synuclein knockdown, the depression manifestations of PD rats were reduced, striatal dopamine and tyrosine hydroxylase levels as well as superoxide dismutase activity were significantly increased, but the levels of reactive oxygen species, malondialdehyde, nitric oxide synthase and nitrogen monoxide were decreased, and simultaneously, the number of apoptotic cells in the striatum was significantly decreased.
The authors considered α-synuclein has the capacity to reverse methamphetamine-induced apoptosis of dopaminergic neurons in the rat striatum by inhibiting oxidative 1 and improving dopaminergic system function. Related results were published in Neural Regeneration Research (Vol. 9, No. 9, 2014).
Article: " Protective effect of alpha-synuclein knockdown on methamphetamine-induced neurotoxicity in dopaminergic neurons," by Yunchun Tai1, Ling Chen1, Enping Huang1, Chao Liu1, 2, Xingyi Yang1, Pingming Qiu1, Huijun Wang1 (1 Department of Forensic Medicine, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong Province, China; 2 Guangzhou Forensic Science Institute, Guangzhou, Guangdong Province, China) Tai YC, Chen L, Huang EP, Liu C, Yang XY, Qiu PM, Wang HJ. Protective effect of alpha-synuclein knockdown on methamphetamine-induced neurotoxicity in dopaminergic neurons. Neural Regen Res. 2014;9(9):951-958.