The effects of Amyloid beta (Aβ)-Aβ-binding alcohol dehydrogenase (ABAD) may exacerbate Alzheimer's disease pathology. Therefore, blocking Aβ-ABAD-mediate effects with ABAD decoy peptide (ABAD-DP) may be a potential therapeutic strategy for Alzheimer's disease.
Protective effect of rAAV/ABAD-DP on H2O2-mediated changes in morphology of PC12 cells. ABAD: Aβ-binding alcohol dehydrogenase; DP: decoy peptide; rAAV: recombinant adeno-associated viral vector; 6His: 6-His protein.
Credit: Neural Regeneration Research
Dr. Jiang Wu and team from the First Hospital of Jilin University in China successfully constructed a recombinant adenovirus constitutively secreting and expressing Aβ-ABAD decoy peptide (rAAV/ABAD-DP-6His).
Their results showed that rAAV/ABAD-DP-6His increased superoxide dismutase activity in hydrogen peroxide-induced oxidative stress-mediated injury of PC12 cells.
Moreover, rAAV/ABADDP-6His decreased malondialdehyde content, intracellular Ca2+ concentration, and the level of reactive oxygen species. rAAV/ABAD-DP-6His maintained the stability of the mitochondrial membrane potential. In addition, the ATP level remained constant, and apoptosis was reduced.
Overall, the experimental findings, published in Neural Regeneration Research, indicate that rAAV/ABAD-DP-6His generates the fusion peptide, Aβ-ABAD decoy peptide, which effectively protects PC12 cells from oxidative stress injury induced by hydrogen peroxide, thus exerting neuroprotective effects.
Article: " rAAV/ABAD-DP-6His attenuates oxidative stress-induced injury of PC12 cells," by Mingyue Jia1, Mingyu Wang2, Yi Yang1, Yixin Chen3, Dujuan Liu4, Xu Wang1, Lei Song1, Jiang Wu1, Yu Yang1 (1 Department of Neurology, the First Hospital of Jilin University, Changchun, Jilin Province, China; 2 Department of Neurology, People's Hospital of Jilin Province, Changchun, Jilin Province, China; 3 Radioactive Medicine Specialty, College of Public Health in Jilin University, Changchun, Jilin Province, China; 4 Department of Burn and Plastic Surgery, the General Hospital of CNPC in Jilin, Jilin, Jilin Province, China)
Jia MY, Wang MY, Yang Y, Chen YX, Liu DJ, Wang X, Song L, Wu J, Yang Y. rAAV/ABAD-DP-6His attenuates oxidative stress-induced injury of PC12 cells. Neural Regen Res. 2014;9(5):481-488.