Identification Of Carbon Dioxide Receptors In Insects May Help Fight Infectious Disease

Main Category: Infectious Diseases / Bacteria / Viruses
Also Included In: Genetics;  Tropical Diseases
Article Date: 17 Dec 2006 - 2:00 PDT

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Mosquitoes don’t mind morning breath. They use the carbon dioxide people exhale as a way to identify a potential food source. But when they bite, they can pass on a number of dangerous infectious diseases, such as malaria, yellow fever, and West Nile encephalitis. Now, reporting in the advance online publication in Nature, Leslie Vosshall’s laboratory at Rockefeller University has identified the two molecular receptors in fruit flies that help these insects detect carbon dioxide. The findings could prove to be important against the fight against global infectious disease.

"Insects are especially sensitive to carbon dioxide, using it to track food sources and assess their surrounding environment," says Vosshall, Chemers Family Associate Professor and head of the Laboratory of Neurogenetics and Behavior at Rockefeller. "The neurons in insects that respond to carbon dioxide were already known, but the molecular mechanism by which these neurons sense this gas was a mystery."

One protein, called Gr21a, was previously known to be expressed in the carbon dioxide responsive neurons, which are in the antennae of the fruit fly. Since in the fly, chemosensory receptors usually work together as a pair of unrelated proteins, Walton Jones, a former biomedical fellow and first author of the paper, began by looking for other members of the gustatory receptor family, and found that the Gr63a protein was always co-expressed with Gr21a, both in the larva and in the adult fly.

"I went on to look at the malaria mosquito and found two homologues of the fly genes, GPRGR22 and GPRGR24. They are also co-expressed in the mosquito’s maxillary palp, the appendage mosquitoes use to sense carbon dioxide," says Jones.

Using genetic manipulation, Jones was able to show that both Gr21a and Gr63a are all that is needed for a fly neuron to sense carbon dioxide. He took neurons that did not normally respond to carbon dioxide and found that only if he expressed both Gr21a and Gr63a together, those neurons now became excited by the gas. He also showed that when Gr63a is mutated, the mutant flies no longer respond to the high levels of carbon dioxide that wild type flies avoid.

These molecules are the first membrane-associated proteins that have been shown to sense a gas. All previously described gas sensors have been cytoplasmic. "Though we don’t know what other proteins might be involved in the signaling pathway, the identification of the carbon dioxide receptor provides a potential target for the design of inhibitors that would act as an insect repellent, "says Vosshall. "These inhibitors would help fight global infectious disease by reducing the attraction of blood-feeding insects to humans."

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This research was supported in part by a grant from the Grand Challenges in Global Health Initiative, the National Institutes of Health, an NIH Medical Scientist Training Program grant, the Jane Coffin Childs Memorial Fund for Medical Research and the Human Frontier Science Program.

Contact: Kristine Kelly
Rockefeller University

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