MicroRNA Targets In C. Elegans Identified By Researchers
Now, researchers at the University of California, San Diego have identified the binding sites of these miRNAs in one of the foremost model organisms, C. elegans, using biochemical means to capture targeted mRNA sequences in vivo.
Argonaute proteins are key players in gene-silencing pathways; miRNAs are anchored into specific binding sites to guide Argonaute proteins to target mRNA molecules for silencing or destruction. By cross-linking interactions between the Argonaute protein bound to miRNA and mRNA duplexes, principal investigators Gene Yeo, PhD, assistant professor in UCSD's Department of Cellular and Molecular Medicine and Amy Pasquinelli, PhD, associate professor in UCSD's Division of Biological Sciences, were able to globally identify their specific binding sites in the nematode.
"Our results were very surprising in that we discovered that individual miRNAs can interact with their targets very differently, and differently than we had expected," said Yeo. "This approach, and the computational analyses that were develop, open up new ways to identify individual miRNA targets in any tissue and cell type in almost any organism."
"The revelation of thousands of endogenous miRNA target sites provides an unprecedented wealth of data for understanding how miRNAs regulate specific targets in a developing animal," added Pasquinelli.
Their work was published online in advance of print on January 10 by Nature Structural & Molecular Biology.
Source: Debra Kain
University of California - San Diego
There are no references listed for this article.
Please use one of the following formats to cite this article in your essay, paper or report:
Kain, Debra. "MicroRNA Targets In C. Elegans Identified By Researchers." Medical News Today. MediLexicon, Intl., 12 Jan. 2010. Web.
27 Jun. 2017. <http://www.medicalnewstoday.com/releases/175648.php>
Kain, D. (2010, January 12). "MicroRNA Targets In C. Elegans Identified By Researchers." Medical News Today. Retrieved from
Please note: If no author information is provided, the source is cited instead.
Contact our news editors
For any corrections of factual information, or to contact our editorial team, please see our contact page.
Copyright Medical News Today: Excluding email/sharing services explicitly offered on this website, material published on Medical News Today may not be reproduced, or distributed without the prior written permission of Medilexicon International Ltd. Please contact us for further details.